High TRIM16 expression correlates with poor prognosis in pancreatic cancer patients. (A, B) Determination and quantification of TRIM16 mRNA levels in pancreatic cancer tissues and paired normal tissues by qRT-PCR (*P<0.05). (C, D) Determination and quantification of TRIM16 protein levels in pancreatic cancer tissues and paired normal tissues by western blotting (*P<0.05). (E, F) Representative image (E) and quantification (F) of IHC staining of TRIM16 in pancreatic cancer tissues and paired normal tissues. Magnification, 100 ×, scale bar, 200 μm; magnification, 400 ×, scale bar, 50 μm. **P<0.01. (G, H) Kaplan-Meier plots representing probabilities of overall survival and disease-free survival in 96 pancreatic cancer patients according to expression level of TRIM16. Statistical analysis was conducted using Student’s t-test and Log Rank test.