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. 2022 Nov 15;12(11):5205–5225.

Figure 4.

Figure 4

TRIM16 enhances glycolysis by increasing SIX1 expression in pancreatic cancer. A. Heat map of top 10 differentially regulated proteins in the glycolysis set in shNC and shTRIM16 cells. B. Protein levels of SIX1 in PANC-1 cells stably transfected with the TRIM16-overexpressing vector. C. Protein levels of SIX1 in AsPC-1 cells transfected with TRIM16-silenced vector. D. After knocking down SIX1 in TRIM16-overexpressing PANC-1 cells, the protein levels of TRIM16 and SIX1 were detected. Tubulin was used as a loading control. E-H. Glucose consumption, lactate production, ATP levels and glycolytic rate and capacity were measured. Three independent experiments were performed. *P<0.05. I. protein levels of GLUT1, HK2, PGK1 and LDHA were detected. Tubulin was used as a loading control. J, K. cell migration and invasive capacity were measured using the transwell assay. *P<0.05. L. After overexpression of SIX1 in TRIM16-silenced AsPC-1 cells, the protein levels of TRIM16 and SIX1 were detected. Tubulin was used as a loading control. M-P. Glucose consumption, lactate production, ATP levels and glycolytic rate and capacity were measured. Three independent experiments were performed. *P<0.05. Q. protein levels of GLUT1, HK2, PGK1 and LDHA were detected. Tubulin was used as a loading control. R, S. cell migration and invasive capacity were measured using the transwell assay. *P<0.05.