Table 3.
100 potential binding proteins of aiphanol from SPR in combination with HPLC-MS
| Protein | Score | Protein | Score | Protein | Score |
|---|---|---|---|---|---|
| STK4 | 1894 | SIRT1* | 1180 | CP19A | 606 |
| Syk | 1872 | NF1* | 1158 | GEMI | 579 |
| VRK1* | 1808 | ANDR* | 1147 | M3K5 | 553 |
| GCR* | 1781 | KMT2A* | 1125 | ATF6A | 528 |
| PGDH | 1769 | NEUR2 | 1122 | IMPA1* | 502 |
| NEK2 | 1730 | OPRM | 1094 | ACES | 477 |
| AAPK2 | 1708 | FYN | 1090 | OXSR1 | 469 |
| BACE1 | 1700 | MK01 | 1063 | SMN | 463 |
| TSHR | 1685 | NR1H4 | 1057 | POLH* | 456 |
| ABCG2 | 1684 | SENP6 | 1054 | ACE | 394 |
| KGP1* | 1677 | HD | 1044 | P53 | 375 |
| GLCM | 1663 | TNFA* | 1015 | PRKN | 371 |
| UD110 | 1646 | IDHC | 1002 | ATX2 | 317 |
| MRP2 | 1645 | RIOK2 | 996 | LOX15 | 317 |
| TOPK* | 1562 | JUN | 988 | PIN1 | 293 |
| PPBI | 1559 | AL1A1 | 977 | DAPK1 | 286 |
| AA1R* | 1514 | SMAD3 | 927 | NR0B1 | 282 |
| COX1* | 1487 | CDK5 | 890 | CP3A4 | 282 |
| GSTO1 | 1462 | ERR1 | 877 | RS4X | 281 |
| AK1C4 | 1457 | GNAI1* | 874 | TCPG | 274 |
| KPCA* | 1457 | NF2L2* | 870 | MBNL1* | 265 |
| TF65 | 1436 | KC1G1 | 866 | RL27 | 259 |
| IL1B | 1419 | LMNA* | 850 | K1C14 | 255 |
| NFKB1* | 1403 | CTIP* | 837 | CPSM | 250 |
| KCC1D | 1402 | CLK1 | 800 | RS14 | 231 |
| CDK16 | 1398 | CFTR | 777 | GRP75 | 188 |
| CHLE* | 1388 | STK16 | 774 | ACTB | 187 |
| ALR | 1381 | ERK1* | 725 | FLNA | 175 |
| PIM3 | 1287 | AHR | 689 | GRP78 | 159 |
| KCMA1 | 1281 | HBB | 664 | MATR3 | 105 |
| UD11 | 1251 | HIF1A | 648 | K2C6A | 75 |
| FLT3 | 1224 | DHB2* | 639 | CSRP1 | 54 |
| GEM | 1207 | GBRA1* | 627 | IQGA1 | 17 |
| FEN1 | 1195 |
*
The binding protein was only present in HCT116 and HT29 cells, but not in SW480 cells.