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. 2022 Nov 15;12(11):5004–5018.

Figure 1.

Figure 1

Methionine restriction inhibits the proliferation of glioma cells in vitro and in vivo. A. Survival of tumor-bearing mice after different feeding programs. B. Representative H&E-stained brain sections of transplanted tumors. C. Expression of Ki67 protein in glioma after mice were fed with or without methionine. Bar: 200 μm. D. U251 and LN229 cells were cultured with different media and cell viability was assessed by a CCK-8 assay. E and F. EdU assay results of U251 and LN229 cells. Bar: 200 μm. G and H. Flow cytometry results of U251 and LN229 cells. ns = nonsignificance, *P < 0.05, **P < 0.01, ***P < 0.001.