Figure 3.

Phylogenetic tree of maize ZmPR-1 gene and PR-1 gene of other plants. A total of 85 spieces including Zea mays (17 pieces, marked with a red dot), Sorghum bicolor (14 pieces, marked with a blue dot), Oryza sativa (32 pieces, marked with a yellow dot) and Arabidopsis thaliana (22 pieces, marked with a green dot) were included for PR-1 protein sequence analysis, using MEGA6.0 software. The Neighbor-Joining (NJ) method of genetic distance was used to construct a phylogenetic tree, the phylogenetic tree was self-checked, and the verification parameter bootstrap value was set to 1000.