. 2022 Nov 15;14(11):7831–7841.

Table 1.

Genes and primer sequences for detection of mutations associated with antibiotic resistance

Antibiotic	Gene	Mutation site	Primer sequences (5’-3’)	Product size
CAM	23S rRNA	A2142; A2143	F: AATGGCGTAACGAGATGGGAG	501 bp
CAM	23S rRNA	A2142; A2143	R: TCCATAAGAGCCAAAGCCCTTAC	501 bp
AMX	PBP1	320Ala; 366Phe; 369Ala; 374Val; 414Ser; 423Leu; 556Thr; 562Asn; 593Thr	F: GAAAAAATCGCTAAAGAAGAGCCAA	851 bp; 549 bp
AMX	PBP1		R: TACGCATGAAATACGAATACACAGG	851 bp; 549 bp
FUR	porD; oorD	G353A; A356G; C357T; A41G; A122G; A335G	F: GGCGTGGATTATTCTCATTGTAAAGG	427 bp; 789 bp
			R: GGATAGGCTGCGATGACATCAATT
			F: CATGCTTTCAGCGCGACTTATA
			R: CCCACTTCAATCGCCGCTTTA
TCN	16S rRNA	AGA926-928	F: GTGCAAGCGTTACTCGGAATCA	366 bp
TCN	16S rRNA	AGA926-928	R: AACCCAACATCTCACGACACGA	366 bp
LEV	gyrA	N87I; N87K; D91N; D91Y; D91G; N87Y; N87T; N87H	F: ATTCCATGAGCGTGATCATAGG	602 bp
LEV	gyrA	N87I; N87K; D91N; D91Y; D91G; N87Y; N87T; N87H	R: GTTATCGCCATCAATAGAGCCAAA	602 bp
MNZ	rdxA	A61; T62; A91; C92; G392; A610; C92; A614	F: TGAGCATGGGGCAGATTTTAAGC	959 bp
MNZ	rdxA	A61; T62; A91; C92; G392; A610; C92; A614	R: TTGAAAACACCCCTAAAAGAGCG	959 bp

AMX, amoxicillin; CAM, clarithromycin; LEV, levofloxacin; MNZ, metronidazole; TCN, tetracycline; FUR, furazolidone.