FIG. 2.

Intracellular AAs available to chlamydiae (strain 434) following graded reduction in medium AA concentrations (100, 75, 40, 25, and 0%), in the presence (open bars) and absence (shaded bars) of CH. Cells were 80% infected with organisms and incubated in medium containing 100 to 0% AAs. Samples were analyzed for AA pools at 20 h p.i. Data for each AA are presented at each medium AA concentration in the order of 100, 75, 40, 25, and 0%. Values are means + SD for triplicate samples, and a separate experiment gave similar results. The total AA pools for 100, 75, 40, 25, and 0% medium AAs in the presence of CH were as follows, in nanomoles per microliter of cell water: 101 ± 5.3, 103 ± 2.5, 72 ± 4.7, 57 ± 6.1, and 16 ± 3.8, respectively; those in the absence of CH were 76 ± 9.9, 67 ± 4.7, 44 ± 1.7, 36 ± 3.5, and 14 ± 1.1, respectively.