TABLE 1.

Morphology and infectivity yield of chlamydiae (strain 434) during decrease in supplied medium AA concentration^a

% Medium AAs	No. of cells infected (IFU/field)b		Yield (IFU/inclusion)
	−CH	+CH	−CH	+CH
100	9 ± 0.18c	23 ± 0.95c	1,278 ± 57	1,938 ± 95
75	14 ± 0.9d*	23 ± 0.47c	372 ± 34*	1,919 ± 37
40	29 ± 2.4e*	23 ± 0.87c	34 ± 4.5*	753 ± 86*
25	48 ± 1.7e*	23 ± 0.87d	19 ± 2.4*	316 ± 42*
0	62 ± 4.2e*	20 ± 0.74e	0.10 ± 0.01*	0.15 ± 0.01*

^aInoculated monolayers were incubated with medium having AA concentrations of 100, 75, 40, 25, or 0% compared with that in CMEM, in presence or absence of CH. Inclusion morphology (electron microscopy) and infectivity yield were assessed at 40 h p.i. Values are means ± SD (at least 10 fields of view in three replicate wells). A separate experiment gave similar results. *, significantly different from control (P < 0.05). 

^bMean number of cells per field = 124. 

^cInclusions with normal morphology and packed with EBs. 

^dInclusions relatively full but particles larger than normal. 

^eInclusions with fewer and markedly abnormal particles.