TABLE 3.
Ratio of intracellular to extracellular AA concentrations in uninfected McCoy cells incubated in plasma medium
| AA(s) | Intracellular/extracellular AA concn ratio in mock-infected cellsa | Rat heart/plasma AA concn ratiob |
|---|---|---|
| Arg | 3.7 | 0.82 |
| Cys | 0.85 | 0.9 |
| Gln | 35 | 9.3 |
| His | 11 | 4.4 |
| Ile | 10 | 1.3 |
| Leu | 8.2 | 1.2 |
| Lys | 2.7 | 1.4 |
| Met | 45 | 2.5 |
| Phe | 10 | 17 |
| Thr | 21 | |
| Trp | 0.75 | |
| Tyr | 10 | 1.7 |
| Val | 6.6 | 0.88 |
| Ala | 28 | 6.1 |
| Asn + Glu | 67 | 48c |
| Asp | 378 | 210 |
| Gly | 32 | 4.5 |
| Pro | 26 | 17 |
| Ser | 25 | 6.9 |
Monolayers were incubated in plasma medium for 4 h before mock infection. Medium was replenished every 6 h, and duplicate AA analyses of both medium and cells were performed at 20 h p.i., enabling calculation of the ratio of the intracellular to the extracellular concentration.
The rat heart tissue/plasma ratios are shown for comparison (4).
Glu only.