TABLE 3.
Ratio of intracellular to extracellular AA concentrations in uninfected McCoy cells incubated in plasma medium
AA(s) | Intracellular/extracellular AA concn ratio in mock-infected cellsa | Rat heart/plasma AA concn ratiob |
---|---|---|
Arg | 3.7 | 0.82 |
Cys | 0.85 | 0.9 |
Gln | 35 | 9.3 |
His | 11 | 4.4 |
Ile | 10 | 1.3 |
Leu | 8.2 | 1.2 |
Lys | 2.7 | 1.4 |
Met | 45 | 2.5 |
Phe | 10 | 17 |
Thr | 21 | |
Trp | 0.75 | |
Tyr | 10 | 1.7 |
Val | 6.6 | 0.88 |
Ala | 28 | 6.1 |
Asn + Glu | 67 | 48c |
Asp | 378 | 210 |
Gly | 32 | 4.5 |
Pro | 26 | 17 |
Ser | 25 | 6.9 |
Monolayers were incubated in plasma medium for 4 h before mock infection. Medium was replenished every 6 h, and duplicate AA analyses of both medium and cells were performed at 20 h p.i., enabling calculation of the ratio of the intracellular to the extracellular concentration.
The rat heart tissue/plasma ratios are shown for comparison (4).
Glu only.