TABLE 4.

Morphology and infectivity yield of chlamydiae (strains 434 and DK20) during glucose deprivation and reversibility of abnormal development^a

Incubation medium	No. of cells infected (IFU/field)b		Yield (IFU/inclusion)
	Strain 434	Strain DK20	Strain 434	Strain DK20
With CH
CMEM	21 ± 1.1c	13 ± 0.7c	5,102 ± 517	640 ± 65
Glucose-free medium	20 ± 1.5d	13 ± 0.8d	17 ± 0.6*	0.15 ± 0.02*
Glucose-free medium (24 h), then CMEM	22 ± 0.8c	ND	3,850 ± 163	ND
Without CH
CMEM	6.4 ± 0.3c	10 ± 0.6c	4,568 ± 417	367 ± 44
Glucose-free medium	34 ± 0.3d*	29 ± 2.1d*	0.63 ± 0.09*	0.01 ± 0.001*
Glucose-free medium (24 h), then CMEM	35 ± 2.0c*	ND	2,424 ± 88*	ND

^aMonolayers inoculated with strain 434 or DK20 were incubated in medium lacking glucose, with or without CH. After 24 h glucose was introduced to devoid monolayers by addition of CMEM (434 only). Morphology (fluorescence microscopy) and infectivity yield were assessed at 40 h p.i. Values are means ± SD (at least 10 fields of view in three replicate wells). *, significantly different from control (P < 0.05). ND, not determined. 

^bMean number of cells per field = 121. 

^cInclusions with normal morphology and packed with EBs. 

^dInclusions with fewer and markedly abnormal particles.