HBSS-H
| Reagent | Final concentration | Amount |
|---|---|---|
| Hanks’ Balanced Salt Solution, 1× (HBSS) | – | 19.8 mL |
| HEPES solution, 1 M | 10 mM | 0.2 mL |
| Total | 20 mL |
Make immediately before dissection, store on ice.
HBSS-H
| Reagent | Final concentration | Amount |
|---|---|---|
| Hanks’ Balanced Salt Solution, 1× (HBSS) | – | 19.8 mL |
| HEPES solution, 1 M | 10 mM | 0.2 mL |
| Total | 20 mL |
Make immediately before dissection, store on ice.