1% FBS/DMEM/PS

Reagent	Final concentration	Amount
Dulbecco’s Modified Eagle’s Medium (DMEM) 1×	–	48.5 mL
Heat-inactivated Fetal Bovine Serum (FBS)	1%	0.5 mL
Penicillin-Streptomycin Solution, 50×	1×	1 mL
Total		50 mL

Store at 4°C, stable for 3 months.