Supplemental Fig. S2ERG28 KO cells show a reduction in total neutral lipid synthesis compared to wild-type cells. The full plate exposure of Fig. 4B with amplified contrast to visualize faint bands. Wild-type or ERG28 KO cells were pretreated for 16 h in sterol deficient media supplemented with 5 μM compactin with or without 10 μM 17-OHP and then radiolabeled with 1 μCi of [¹⁴C]-acetate for 4 h in sterol deficient media with or without 10 μM 17-OHP. Total nonsaponifiable sterols were harvested from the cells, dried and separated on TLC Silica gel 60 F₂₅₄ plates in a heptane: ethyl acetate (2:1) mobile phase. Radiolabeled sterols were imaged after 1 week of exposure using the Typhoon FLA 9500 phosphor imager