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. 2022 Nov 12;298(12):102696. doi: 10.1016/j.jbc.2022.102696

Figure 1.

Figure 1

8A selectively inhibited IL-1β secretion.A, chemical structure of 8A. B, PMA-differentiated THP1 cells were treated with 8A with the indicated concentrations. After 24 h, cell viability was determined by MTT assay. C, PMA-differentiated THP1 cells and (D) BMDMs were stimulated with 100 ng/ml LPS for 3 h, followed by indicated concentrations of 8A treatment for 1 h and then another 1 h of 5 mM ATP stimulation. IL-1β and TNF-α in supernatants were determined by ELISA. E, PMA-differentiated THP1 cells and (F) BMDM were stimulated with 100 ng/ml LPS for 3 h, followed by indicated dose of 8A treatment with or without 500 μg/ml MSU for 2 h. IL-1β and TNF-α in supernatant were determined by ELISA. Andro: andrographolide. Data were presented as mean ± SD of three independent experiments. ∗p < 0.05, ∗∗p < 0.01 versus LPS + ATP. BMDMs, bone marrow-derived macrophages; LPS, lipopolysaccharide; MSU, monosodium urate; PMA, phorbol myristate acetate.