Figure 8.

8A alleviated MSU-induced peritonitis and arthritis.A and B, mice were i.g. with indicated doses of 8A for 3 days (once a day) before i.p. injection of MSU (1 mg MSU crystals dissolved in 0.5 ml sterile PBS). After 6 h, the mice were euthanized, and the peritoneal cavities were lavaged with 3 ml cold PBS. Peritoneal exudate cells (PECs) were counted, and the expression of CASP1 in PEC was determined by Western blotting. C–E, foot paw thickness changes were measured at different times after MSU injection (C). After MSU injection for 6 h, the manifestation of foot paws was recorded (D) and subjected to H&E staining (E). F, CASP1 p20 expression was analyzed by immunochemical staining. Data in A and C were presented as the mean ± SD of six mice. Data in B were presented as the mean ± SD of four mice. Data in E and F were expressed as the mean ± SD of five fields per mouse in each group. ∗p < 0.05, ∗∗p < 0.01 versus MSU group. Scale bar 100 μm in E and F. MSU, monosodium urate.