Figure 4.

Splenocytes from unisexually infected mice produce Th1 and Th2 cytokines following SWAP stimulation. Splenocytes were isolated from unisexually male-infected, unisexually female-infected and uninfected (naive) control mice and stimulated with 10 µg/ml soluble worm antigen preparation (SWAP). Supernatants were collected after 72 hours and amounts of IFN-γ, IL-4, IL-13 und IL-10 were quantified by ELISA (n=4-6). Data are presented as mean ± SEM from duplicate data. P values < 0.05 were considered statistically significant. *p < 0.05, **p < 0.01.