TABLE 2.
Antimicrobial activity of 14 endophytic fungi isolated from Nervilia fordii against human pathogenic microorganisms by agar well diffusion method.
| Isolate No. | Classification | GenBank No. | Closest match (GenBank No.)−% seq identity | Antimicrobial activitya |
||
| Escherichia coli | Staphylococcus aureus | Candida tropicalis | ||||
| 1,151 | P. macrosclerotiorum | MZ400540 | P. macrosclerotiorum (MH863005.1)−99.06% | 34.70 ± 3.36 | 28.48 ± 0.74 | – |
| 1,154 | E. sorghinum | MZ400542 | E. sorghinum (FJ427071.1)−99.57% | 22.12 ± 0.78 | 15.70 ± 1.09 | – |
| 1,174 | Sordariomycetes sp. | OK042827 | S. eucalypti (NR_170747.1)−89.39% | – | 9.66 ± 0.34 | – |
| 1,198 | B. sacchari | MZ400565 | B. sacchari (KJ830829.1)−99.27% | 12.06 ± 2.08 | 12.52 ± 0.86 | – |
| 1,211 | A. alternata | MZ400575 | A. alternata (MH864614.1)−100% | – | 8.67 ± 0.19 | – |
| 1,225 | F. oxysporum | MZ400582 | F. oxysporum (JF807397.1)−99.43% | – | 9.95 ± 0.54 | 12.11 ± 0.63 |
| 1,230 | T. blackeriella | MZ400584 | T. blackeriella (NR_159037.1)−99% | 9.96 ± 0.04 | – | – |
| 1,232 | Arthrinium sp. | MZ400585 | A. subroseum (NR_157471.1)−97.24% | – | 8.74 ± 0.55 | – |
| 1,238 | A. versicolor | MZ400590 | A. versicolor (MH865273.1)−99.62% | 11.08 ± 1.02 | 10.27 ± 0.82 | – |
| 1,193 | A. amoenus | MZ400564 | A. amoenus (OL772676.1) −99.81% | 8.52 ± 0.46 | – | – |
| 1,260 | Collariella sp. | MZ400601 | C. carteri (MH861860.1)−96.17% | – | 8.73 ± 0.24 | – |
| 1,262 | Lecanicillium sp. | MZ400603 | L. araneicola (NR_121208.1)−96.43% | 9.14 ± 0.61 | 9.04 ± 0.71 | – |
| 1,303 | S. cycadis | MZ400622 | S. cycadis (MH863303.1)−99.85% | 11.08 ± 0.87 | – | 9.84 ± 0.55 |
| 1,307 | S. kiliense | MZ400625 | S. kiliense (MH859733.1)−99.25% | – | 10.58 ± 1.70 | – |
| Streptomycin (5 mg ml–1, 20 μL) | 24.477 ± 2.48 | 23.34 ± 1.85 | – | |||
| Tetracycline (5 mg ml–1, 20 μL) | 26.44 ± 1.74 | 24.85 ± 3.13 | – | |||
aAntimicrobial activity illustrated by diameter of inhibition zone, “–” means antibacterial activity not been detected. Streptomycin (SM) and Tetracycline (TET) are used as the positive control.