Figure 3.

In vitro anti-inflammatory actions of PL8177 and αMSH on mouse primary macrophages. Biogel-elicited peritoneal macrophages were incubated for 30 min with Melanocortin compounds prior stimulation with zymosan (Zym, 25μg/ml) for further 6 h. (A) Gene expression for inflammatory related genes was measured by real time-PCR on cells treated with vehicle (Veh) or with 2μM of PL8177 or αMSH. Unstimulated cells were used as control for normalization. Fold changes (FC) were calculated as 2^(-ΔΔCt), using Gapdh as reference gene (B) Summary of gene expression measurements, including genes that were not significantly altered by the treatments, expressed as log₂FC. (C) Concentration-response effect of PL8177 and αMSH (0.08-10μM) on cytokine release by macrophages measured by ELISA. For all experiments, 5 mice were used and analyzed by repeated measures one-way ANOVA followed by multiple comparison test. Data represent mean ± SEM. *p<0.05 vs. zymosan alone (Zym).