Figure 4.

Pro-resolving effects of PL8177 on Macrophage efferocytosis. Biogel-elicited peritoneal macrophages were incubated with Melanocortin compounds (2μM) for 30 min prior to the addition of apoptotic neutrophils (at a Macrophage:neutrophil ratio of 1:5). After 1 h, cells were stained using the myeloperoxidase (MPO) assay to visualize ingested neutrophils. (A) Representative image shows the dark brown coloration selectively acquired by engulfed neutrophils. An example of single and a multiple ingestion are indicated. (B) Total phagocytosis was calculated as % of macrophages containing at least one neutrophil inside. (C) Multi-phagocytic cells containing more than 1 neutrophil were also quantified. These data show the percentage of macrophages that have ingested more than one neutrophil, with respect to the total Macrophage population (D) Clearance Index was calculated as (% phagocytic cells x % multiple ingestions) x100. Data represent mean ± SEM of 5 mice analyzed by repeated measures one-way ANOVA followed by multiple comparison test. *p<0.05, **p<0.01 vs. untreated (Veh).