Figure 6.

Effect of PL8177 in immune cell recruitment to arthritic joints. Flow cytometry analysis of hind paw cells of arthritic vehicle-treated (Veh) and PL8177-treated (3 mg/kg) mice was performed. Total cell numbers were obtained by normalization with quantification beads added upon enzymatic digestion. The gating strategy is provided in Supplementary Figure 1 .The different populations were defined as follows: total myeloid cells (CD45⁺ CD11b⁺), neutrophils (CD45⁺ CD11b⁺ Ly6G⁺), mono-macrophages (CD45⁺ CD11b⁺ SiglecF^- Ly6G^-), monocytes (CD45⁺ CD11b⁺ SiglecF^- Ly6G^hi F4/80^lo), macrophages (CD45⁺ CD11b⁺ SiglecF^- Ly6G^lo F4/80^hi), eosinophils (CD45⁺ CD11b⁺ SiglecF⁺), T cells (CD45⁺ CD11b^- B220^- CD3e⁺), B cells (CD45⁺ CD11b^- B220⁺). The mono/Macrophage population was also analysed based on Ly6C and MHC-II markers. Data are mean ± SEM of n=7 analyzed by t-test. *p<0.05 vs. vehicle-treated arthritic mice (Veh).