FIG. 2.

A ToxR-dependent ∼38-kDa protein is overexpressed during growth of classical and El Tor V. cholerae strains in bile and DC. Whole-cell lysates of V. cholerae classical strains (left panel) O395 (wild type) and KKV61 (ΔtoxR) and El Tor strains E7946 (wild type) and KKV366 (ΔtoxR) grown in LB alone, LB plus 0.4% bile, or LB plus 0.1% DC, as indicated above lanes. Samples were matched by equivalent OD₆₀₀ units, separated by SDS–10% PAGE, and stained with Coomassie blue. The first lane of each panel has molecular mass markers, which are noted in kilodaltons to the left. The known mobilities of OmpU and OmpT are indicated by arrows.