Fig. 3.

Prediction and demonstration of CREB1 as the direct target of miR-125b-5p. A Bioinformatic analysis for miR-125b-5p binding to CREB1 3′UTR. B Luciferase reporter plasmids carrying CREB1 3'UTR and wild-type/mutant miR-125b-5p binding regions were transfected with the same amount of control mimic or miR-125b-5p mimic constructs. C–F After transfecting A549 cells with miR-125b-5p mimic/inhibitor, CREB1 protein expression was quantified by western blotting (C, D); miR-125b-5p and CREB1 mRNA expression levels were analyzed by qRT-PCR (E, F). G and H The protein expression of CREB1 was quantified by western blotting for the following conditions: a. control mimic + control vector; b. control mimic + CREB1 vector; c. miR-125b-5p mimic + control vector; d. miR-125b-5p mimic + CREB1 vector. **p < 0.01, ***p < 0.001