Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 24;4:1033581. doi: 10.3389/frph.2022.1033581

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Maurya, Szwarc, Lonard, Gibbons, Wu, O'Malley, DeMayo and Lydon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Expression levels of SRC-3 do not significantly change during T-HESC decidualization. (A) Schematic summarizes the experimental design of EPC decidualization of T-HESCs in culture. Following culture in standard culture medium for forty-eight hours, T-HESCs were switched to EPC medium to elicit decidualization over time. Cells were harvested at 0, 1, 2, 4, and 6 days following EPC administration for qPCR and western analysis. (B) As expected, the established PRL biomarker for decidualization (43) is significantly induced following EPC administration with time. (C) The expression levels of SRC-3 do not markedly change from day 0 to day 6 of EPC treatment. (D) The results in (C) are confirmed at the protein level by western analysis, which show there is minimal change in the levels of SRC-3 protein during T-HESC decidualization. (E) From a publicly available GEO dataset [GSE: 104721 (44)], raw transcript scores for SRC-3 (NCOA3) were calculated from HESCs cultured at day 0 (non-decidualized) and day 4 (decidualized) in deciduogenic medium. Note that SRC-3 transcript levels in T-HESCs do not significantly change following decidualization; n = 3 samples per treatment group. (F) The SRC-3 raw transcript scores from endometrial RNA obtained from women diagnosed with RPL compared with patients diagnosed with no RPL (control) (45); endometrial tissue was biopsied at the mid-secretory phase of the cycle. Original transcript data were obtained from the publically available RNA-seq dataset [GEO accession: GSE 65099 (45)]. Analysis reveals that SRC-3 RNA is significantly increased in endometrial tissue biopsied from RPL patients when compared to control patients. Results are displayed as the mean ± SE; n = 10 women per RPL and control groups.