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. Author manuscript; available in PMC: 2022 Dec 8.
Published in final edited form as: J Vis Exp. 2021 Jun 30;(172):10.3791/62768. doi: 10.3791/62768

Figure 6: A549 cells internalize NHF-ATP along with HMWFD in vivo.

Figure 6:

Fluorescence microscopy of tumorigenic A549 cells, xenografted into immunodeficient (Nu/J) mice; NHF-ATP internalization was performed in vivo with 8 mg/mL HMWFD and 100 μM NHF-ATP being directly injected into tumors of living mice. Co-localization of cellular HMWFD and NHF-ATP is shown as merged images in A and B (right panels, yellow). (A) High-magnification images highlight cellular internalization of NHF-ATP and HMWFD in tumors in vivo. Scale bars = 20 μm. (B) Low-magnification images depict regional internalization within a tumor tissue section. Scale bars = 100 μm.