FIG. 6.

Blocking of lymphocyte adherence in the upper and lower regions of the GT. MLN cells from mice infected with MoPn were harvested on day 7. Monoclonal antibodies against murine VCAM-1, ICAM-1, MAdCAM-1, and an isotype-matched control antibody were incubated on freshly cut GT frozen sections for 30 min at room temperature. Single cell suspensions (2 × 10⁶ cells) were applied to freshly cut frozen GT sections from the CV region on day 7 (A) or the UH plus OD regions on day 14 (B). Adherent lymphocytes from 10 to 15 venules per section were counted. Cells from multiple sections for each time point were counted. Each data point represents the mean ± standard error of the mean (SEM) of the number of adherent lymphocytes from 90 to 139 individual venules. ∗, P < 0.05 by ANOVA and Dunnett's (A) and Dunn's (B) post-hoc test.