TABLE 1.
Leukocyte recruitment to different regions of the GT throughout the course of MoPn infection
| Cell type and region | Leukocyte recruitmenta on day:
|
|||
|---|---|---|---|---|
| 0 | 7 | 14 | 21 | |
| CD4b | ||||
| CV | 3.1 (0.1) | 11.9 (7.1) | 26.1 (6.4) | 5.4 (2.1) |
| UH | 6.7 (4.7) | 25.1 (1.4) | 30.3 (2.1) | 48.2 (8.6)c |
| OD | 0.0 | 18.5 (1.9) | 51.8 (2.7)c | 52.5 (12.7)c |
| CD8 | ||||
| CV | 0.0 | 5.2 (7.5) | 13.5 (3.8) | 2.0 (0.9) |
| UH | 0.0 | 3.7 (15.0) | 15.6 (3.8) | 14.9 (0.8) |
| OD | 0.0 | 0.0 | 24.0 (1.9) | 16.2 (4.5) |
| Neutrophil/macrophaged | ||||
| CV | 5.4 (0.3) | 34.9 (9.2) | 54.9 (6.0) | 16.5 (8.1) |
| UH | 11.9 (8.2) | 26.9 (9.0) | 38.2 (6.5) | 30.1 (1.4) |
| OD | 77.4 (70.9) | 153.2 (107.8) | 136.4 (9.9) | 45.5 (8.9) |
| Macrophage | ||||
| CV | 3.5 (0.9) | 1.7 (6.0) | 19.0 (10.1) | 3.1 (0.9) |
| M | 3.4 (2.0) | 3.0 (12.0) | 9.5 (3.7) | 13.3 (4.5) |
| OD | 0.0 | 13.3 (18.7) | 3.8 (4.9) | 10.3 (7.1) |
a
Values are the means (± standard errors of the means) of two independent pools of GT tissue. Data are expressed as the numbers of CD4-, CD8-, CD11b-, or F4/80-positive cells (in thousands) per 106 total cells recovered from collagenase-digested GTs.
b
There was a significant increase in CD4 cells in the UH and OD regions compared to the CV region; P < 0.001 by ANOVA.
c
There was a significant increase in cell numbers compared to day 0 values; P < 0.05 by Tukey's post-hoc test.
d
There was a significant increase in the number of cells on day 14 compared to day 0; P = 0.037 by ANOVA.