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. 2022 Nov 22;61(23):2766–2775. doi: 10.1021/acs.biochem.2c00531

Figure 2.

Figure 2

(A) Increase in vobs/[E] with increasing [EA] for RAdK-catalyzed reactions of 1 mM ATP with phosphite dianion at pH 7.5, I = 0.15 (NaCl), and 25 °C. Key: ◆ 23 mM [HPO32–] and 2 μM RAdK; ▼ 19 mM [HPO32–] and 2 μM RAdK; ▲ 14 mM [HPO32–] and 2 μM RAdK; ■ 9.2 mM [HPO32–] and 1 μM RAdK; ● 4.6 mM [HPO32–] and 1 μM RAdK. (B) Effect of increasing [HPO32–] on the values of (kcat/KAct)obs determined for (A).