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(A) Increase in vobs/[E] with increasing [EA] for HAdK1-catalyzed (4 μM) reactions of 1 mM ATP with FPO32– at pH 7.5, I = 0.15 (NaCl), and 25 °C. Key: ● 25 mM [FPO32–]; ◆ 20 mM [FPO32–]; ▼ 15 mM [FPO32–]; ▲ 10 mM [FPO32–]; ■ 5 mM [FPO32–]. (B) Effect of increasing [FPO32–] on the values of (kcat/KAct)obs determined for (A).
