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(A) Increase in vobs/[E] with increasing [EA] for HAdK1-catalyzed (70 μM) reactions of 1 mM ATP with HOPO32– at pH 7.5, I = 0.15 (NaCl), and 25 °C. Key: ● 17 mM [HOPO32–]; ◆ 13 mM [HOPO32–]; ▼ 10 mM [HOPO32–]; ▲ 6.6 mM [HOPO32–]; ■ 3.3 mM [HOPO32–]. (B) Effect of increasing [HOPO32–] on the values of (kcat/KAct)obs determined for (A).
