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(A) Increase in vobs/[E] with increasing [5′-dAdo] for HAdK1-catalyzed (12 μM) reactions of 1 mM ATP with phosphite dianion at pH 7.5, I = 0.15 (NaCl), and 25 °C. Key: ● 23 mM [HPO32–]; ◆ 19 mM [HPO32–]; ▼ 14 mM [HPO32–]; ▲ 9.2 mM [HPO32–]; ■ 4.6 mM [HPO32–]. (B) Effect of increasing [HPO32–] on the values of (kcat/K5′dAdo)obs determined for (A).
