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. 2022 Nov 22;61(23):2766–2775. doi: 10.1021/acs.biochem.2c00531

Table 3. Third-Order Rate Constants (Scheme 4) for Activation of HAdK1-Catalyzed Phosphoryl Transfer Reactions by EA, Adenosine, and Deoxyadenosinesa.

activator (kcat)XP·Act/KXPKAct
EA 260b
5′-dAdo 34 ± 1.7c
Ado 1.6 ± 0.35d
2′-dAdo not detectede
3′-dAdo not detectedf
a

Determined at pH 7.5 and 25 °C in 25 mM TEA buffer and I = 0.15 (NaCl). The uncertainty in the kinetic parameters is the standard error from the least-squares fits of the kinetic data to eq 3.

b

Published data.23