TABLE 2.
Tryptic peptides of the B. caccae 100-kDa proteina
Peptide | Peak | Purity (%) | Size | Amino acid sequence |
---|---|---|---|---|
1 | 71 | 100 | 1,162.9 | Asp-Pro-Ser-Ser-Leu-Ala-Ile-Phe-Gly-Val-Arg |
3 | 35 | 100 | 1,199.1 | Gly-Pro-Ser-Glu-Ala-Asp-Ala-Phe-Tyr-Asn-Cys |
Tyr | ||||
2 | 45 | 87 | 1,007.7 | Tyr-Phe-Leu-Ser-Ala-Ser-Tyr-Arg-X |
Ala Gly-Asn-Gly-Glu | ||||
Ser |
The protein was isolate by SDS-PAGE from p2Lc3, and tryptic peptides were produced and separated by size exclusion HPLC. The tryptic peptides were analyzed by MALDI-MS for size determination and subjected to Edman degradation for NH2-terminal peptide sequence.