Skip to main content
. 2000 Mar;68(3):1542–1548. doi: 10.1128/iai.68.3.1542-1548.2000

TABLE 2.

Tryptic peptides of the B. caccae 100-kDa proteina

Peptide Peak Purity (%) Size Amino acid sequence
1 71 100 1,162.9 Asp-Pro-Ser-Ser-Leu-Ala-Ile-Phe-Gly-Val-Arg
3 35 100 1,199.1 Gly-Pro-Ser-Glu-Ala-Asp-Ala-Phe-Tyr-Asn-Cys
            Tyr
2 45 87 1,007.7 Tyr-Phe-Leu-Ser-Ala-Ser-Tyr-Arg-X
Ala                 Gly-Asn-Gly-Glu
Ser
a

The protein was isolate by SDS-PAGE from p2Lc3, and tryptic peptides were produced and separated by size exclusion HPLC. The tryptic peptides were analyzed by MALDI-MS for size determination and subjected to Edman degradation for NH2-terminal peptide sequence.