TABLE 1.
Clone | Protein amt (μg/1010 cells) with growth temp (°C) and conditione
|
|||
---|---|---|---|---|
30
|
37
|
|||
Anaerobic | Aerobic | Anaerobic | Aerobic | |
pSBRnirBc | 11.3 | 1.15 | 22.7 | 2.39 |
pSBR-CTA2/BnirBc | 39.8 | 2.24 | 25.9 | 0.25 |
pSBR-CTA2/BnirBd | 30.1 | 0.01 | 23.9 | 4.14 |
Detected with a sandwich ELISA using plates coated with rabbit IgG anti-SBR and developed with rabbit IgG anti-SBR–HRP.
Detected with GM1 ELISA. GM1 ganglioside was used for coating, and rabbit IgG anti-SBR–HRP was used for detection.
Cultures were grown with antibiotic selection.
Cultures were grown without antibiotic selection.
Data are the means of duplicate determinations.