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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: Mol Cell. 2022 Nov 16;82(23):4428–4442.e7. doi: 10.1016/j.molcel.2022.10.025

Figure 6. PAX3-FOXO1 is required for maintaining open chromatin structure at the regulated enhancers.

Figure 6.

(A) Histograms of the CUT&RUN signal of the indicated factors around all PAX3-FOXO1 peaks and the ATAC-seq peaks regulated by PAX3-FOXO1 in Rh30 cells. (B) MA plots showing ATAC-seq peak changes at 0.5 hr, 1hr and 2hr after PAX3-FOXO1 degradation; red (up) or blue (down) (dTAG-47/DMSO, n=2). (C) Heatmap of a short time course of dTAG-47 treatment that uses ATAC-seq changes at 2hr after PAX3-FOXO1 degradation from Rh30 cells. (D) Genome browser view of the RUNX2 “super” enhancer. The shaded box highlights an enhancer showing changes in factor binding, eRNAs, and accessibility over time compared to neighboring enhancers.