FIG. 4.

(A) The supernatant from D39 (■) at 10⁸ CFU/ml shows a time-dependent increase hemolytic activity, whereas PLN-A (□) at 10⁸ CFU/ml shows no hemolytic activity in the supernatant over 60 min at 37°C. ∗, statistically (P < 0.05; paired t test) increased compared with 0 min. (B) Additive action of pneumolysin and H₂O₂ on ependymal CBF (■, control; □, 10 μM H₂O₂; ●, 1 HU of pneumolysin per ml; ○, 10 μM H₂O₂ plus 1 HU of pneumolysin per ml). ∗, statistically (P < 0.05, paired t test) inhibited compared with pneumolysin alone. All data are mean and standard error of the mean of five individual experiments.