| Microscope |
Titan Krios |
Titan Krios |
| Detector |
K2 Summit |
K3 Summit |
| Magnification |
165,000 |
105,000 |
| Voltage [kV] |
300 |
300 |
| Total electron exposure [e−/Å2] |
30–32 |
40 |
| Defocus range [μm] |
−0.2 to −3.6 |
−0.2 to −3.7 |
| Pixel size [Å] |
0.83 |
0.846 |
| Symmetry imposed |
C1
|
C1
|
| Final particle |
885,199 |
899,952 |
| Resolution [Å] (overall/body/shoulder/platform/back/tail/head-AP/head-PE/mS39/mtIF3) |
2.40/–/2.23/2.28/2.29/2.41/2.26/2.39/2.57/2.20 |
2.31/2.28/–/–/–/–/–/–/– |
| Map-sharpening B-factor [Å2] (overall/shoulder/platform/back/tail/head-AP/head-PE/mS39/mtIF3) |
−53/–/−47/−49/−56/−60/−52/−58/−68/−54 |
−50/−49/–/–/–/–/–/–/– |
|
Refinement
|
|
|
| Model composition |
|
|
| Total atoms (non-hydrogen/hydrogen) |
71,880/59,406 |
|
| Chains (RNA/protein) |
1/31 |
|
| RNA residues (non-modified/m4C, m5C, m5U, m62A) |
950/1/1/1/2 |
|
| Protein residues (non-modified/N-acetylAla/O1-methylisoAsp) |
5915/2/1 |
|
| Metal ions (Mg2+/K+/Zn2+) |
62/21/1 |
|
| Ligands (2Fe–2S/ATP/GMPPNP/NAD/spermine/streptomycin) |
2/1/1/1/1/1 |
|
| Waters |
3087 |
|
| Model to map CC (CCmask/CCbox/CCpeaks/CCvolume) |
0.90/0.79/0.79/0.88 |
|
| Resolution [Å] by model-to-map FSC, threshold 0.50 (masked/unmasked) |
2.18/2.18 |
|
| Average B-factor [Å2] (RNA/protein/metal ion and ligand/water) |
36/48/32/32 |
|
| R.m.s. deviations, bond lengths [Å]/bond angles [°] |
0.002/0.408 |
|
|
Validation
|
|
|
| Clash score |
1.39 |
|
| Rotamer outliers [%] |
0 |
|
| Ramachandran plot [%] (favored/ allowed/disallowed) |
98.01/1.95/0.03 |
|
| CaBLAM outliers [%] |
0.71 |
|
|
Cβ outliers [%] |
0 |
|
| MolProbity score |
0.87 |
|
| EMRinger score |
6.16 |
|
| PDB/EMDB accession code |
7P2E/EMD-13170 |
–/EMD-15542 |
