Figure 3. Post-transcriptional regulation mediated by Salmonella enterica and Escherichia coli GlnZ.

Predicted interactions of GlnZ with the target mRNAs are shown in the panels, (A) S. enterica sucA, (B) E. coli sucA, (C) S. enterica glnP, (D) S. enterica deoD, (E) E. coli aceE, and (F) S. enterica aceE. The nucleotide numbers relative to the start codon of the target mRNA and the stop codon of glnA are shown above and below the nucleotide sequences, respectively. The mutated nucleotides are indicated in red, and the extra G nucleotide found in Salmonella GlnZ1 is shown in purple. E. coli ΔglnZ strain was transformed by GFP translational fusion plasmids along with pJV300 control vector or GlnZ expression plasmids (Supplementary file 5-7). Mean relative fluorescence units (RFU) normalized by OD₆₀₀ calculated from biological replicates (n>3) are presented with standard deviation in percentage relative to the vector control. Statistical significance was calculated using one-way ANOVA and denoted as follows: **p<0.005, *p<0.05.

Figure 3—figure supplement 1. Post-transcriptional regulation of sucA involves a purine-rich sequence.

(A) Predicted interaction of GlnZ with sucA mRNA in Salmonella and Escherichia coli. The nucleotide numbers relative to the start codon of sucA (bold) and the stop codon of glnA are shown above and below the nucleotide sequences, respectively. Putative SD sequence of sucA is underlined. The deleted regions in the pXG30-sf sucA reporter plasmids, Δ15, Δ127, and Δ142 are boxed. (B) E. coli ΔglnZ strain was transformed by GFP translational fusion plasmids along with pJV300 control vector or GlnZ expression plasmids (Supplementary file 5-7). Mean relative fluorescence units (RFU) normalized by OD₆₀₀ calculated from biological replicates (n>3) are presented with standard deviation in percentage relative to the vector control of sucAsal. Statistical significance was calculated using one-way ANOVA and denoted as follows: **p<0.005, *p<0.05, NS: not significant.

Figure 3—figure supplement 2. Post-transcriptional regulation of aceE by GlnZ_O157.

(A) Escherichia coli ΔglnZ strain was transformed by pXG30sf-aceE_eco along with pJV300 vector control or GlnZ_O157 expression plasmids (Supplementary file 5-7). Mean relative fluorescence units (RFU) normalized by OD₆₀₀ calculated from biological replicates (n>3) are presented with standard deviation in percentage relative to the vector control. Statistical significance was calculated using one-way ANOVA and denoted as follows: **p<0.005. (B) Alignment of Escherichia fergusonii GlnZ from 55 strains along with GlnZ_O157. GlnZ_O157 is shown in bold font. Nucleotides of E. fergusonii GlnZ different from those of GlnZ_O157 are highlighted in yellow and indicated in red font.