TABLE 1.

Oligonucleotide primers and probes used in this study

Designation	Sequence	Purpose
p21-spec	5′-GCAAGTAATAATGAGTTAAAAGTTAAGCAAAG-3′	Library screening
p21-specR+C	5′-CTTTGCTTAACTTTTAACTCATTATTACTTTGC-3′	Long-distance PCRd
elpB2 5′	5′-GAGATCAATGGAGATATTGATG-3′	Library screening
elpB2 3′	5′-CACCACCCCAAGTTTTGCGTATAG-3′	Library screening
ospF-LD-F	5′-CATCTATAACTTTGTTTGCCAATTCATTGC-3′	Long-distance PCRbc
2.10-LD-F	5′-GGCCAACAACCAGTCTTGTTGTTTATTCGC-3′	Long-distance PCRbc
2.11-LD-F	5′-GCCCCCCGTTGGTTTTGTTCTCTTATTTGC-3′	Long-distance PCRb,c
elpA1-LD-F	5′-GAGCTTTGAGCTTTAATTATTTTGAGCAGCACAAGTAGA-3′	Long-distance PCRbc
elpA2-LD-F	5′-CGGAATTTTTAATATATTTTTACGCTGTC-3′	Long-distance PCRbc
elpB1-LD-F	5′-CGTGAAGTGAAAGTGAAAAAAGTAGAAGTATCCG-3′	Long-distance PCRbc
elpB2-LD-F	5′-CGTGAAGTGAAAGTGAAAAAAGTAGAAGTATCCG-′	Long-distance PCRbc
mlp 5′-cons	5′-AAAGGAGTAACAATGAAAATYATCAAC-3a	Long-distance PCRd
mlp1-spec	5′-GAGACGGTAGTGGCAACTTAATAGAGC-3′	Long-distance PCRd
mlp3-spec	5′-GTTACTACCTGCGGCAATGGTGGTTCC-3′	Long-distance PCRd
mlp5-spec	5′-AAGCTAATACTCCACAAGATTGTAAAAATAA-3′	Long-distance PCRd
mlp7A-spec	5′-CGGTATAGATACTTTCACGGCTCAAAGC-3′	Long-distance PCRd
mlp7B-spec	5′-CAATAGTGTCCAGCAAGCAAAGCAG-3′	Long-distance PCRd
mlp8-spec	5′-ACAGAGCAGTCAGATAGTACATGTGGTG-3′	Long-distance PCRd
mlp9-spec	5′-AATCCAAACAAATGTAATGATAATAAACATGG-3′	Long-distance PCRd
mlp10-spec	5′-CCACATACAAAACTTTAGTTAAAGAATCTCT-3′	Long-distance PCRd
cp32-1 BBP41cons(R+C)	5′-TCGCTTCTAACTTTCCTAGCGGTTAACTTCTG-3′	Long-distance PCRc
cp32cons (4306)R+C	5′-CTCGCTCCATAACACATAATGCAGTGTTA-3′	Long-distance PCRb
cp32cons (8952)R+C	5′-AAGTCTTACCACTTCAGAACTAAATCCA-3′	Long-distance PCRb
cp32cons (11813)R+C	5′-ACAACAAAAGTGTGTCTATGTACTTTAAGA-3′	Long-distance PCRb
cp18/32 (8435) 3′ LD	5′-TTTCTCTTAAGTATTTAACCTCTTAAGCTTTAAAACAGC-3′	Long-distance PCRb

^a Y, nucleotides C or T. 

^b Deletion analysis. 

^c Variable-locus sequence analysis. 

^d mlp and ospE/ospF/elp linkage analysis.