Fig. 1.

Inhibition of RV replication by 2-DG in HeLa Ohio cells and HNECs. Intracellular viral RNA was assessed by qPCR 7 h post-infection at 0.005 TCID₅₀/cell for the indicated RV strains in HeLa Ohio cells in medium containing 1 g/L glucose (A). Comparison of IC₅₀ of 2-DG on the indicated RV strains under physiological versus conventional culture conditions (B). Intracellular viral RNA was assessed by qPCR 7 h post-infection at 4.5 × 10⁴ TCID₅₀/well for the indicated RV strains in HNECs (C). In (A) and (C) cells were treated with the indicated concentrations of 2-DG (represented on a log10 scale) 1 h post-infection until samples were collected. The viability of HNECs was assessed at 7 h post-treatment with indicated concentrations of 2-DG (D). Graphs show pooled result ± SEM of 3–4 independent experiments. HNEC: human nasal epithelial cells, RV: rhinovirus.