Fig. 2.

Intracellular storage of 2-DG6P after short-term exposure to 2-DG. 2-DG uptake experimental setup (A). Luminescence-based measurements of intracellular 2-DG6P at the indicated times after HeLa Ohio cells (B) or HNECs (C) were exposed to 2-DG for 10 min. In (B) and (C), the left graphs show the 2-DG6P levels (in RLU) at time 0 min (i.e., immediately after 10 min 2-DG treatment), and the right graphs show percentage decay of 2-DG6P over time in HeLa Ohio and HNECs, respectively. Data show pooled result ± SEM of 2–3 independent experiments. RLU: relative luminescence units, HNEC: human nasal epithelial cells.