Figure 1.

Src phosphorylates hYVH1 on residue Tyr 179.A, HeLa cells expressing FLAG-hYVH1 and myc-Src Y530F were lysed and subjected to FLAG immunoprecipitation and immunoblot analysis. Antiphosphotyrosine (4G10) blot depicting an increase in hYVH1 tyrosine phosphorylation when coexpressed with Src is shown (top panel). Boxed lines on blots indicate where irrelevant sample lanes were cropped out to simplify blots and line up relevant samples. B, schematic representation of the hYVH1 sequence showing the location of the mapped phosphorylation site Tyr¹⁷⁹ with respect to its domain structure. C, multiple sequence alignment of the tryptic peptide corresponding to amino acid residues 163 to 180 of hYVH1. Regions of conservation across species are highlighted including the phosphorylation site Tyr¹⁷⁹. hYVH1, human YVH1.