Figure 1.

SEC-MALS analysis of the α-Q139A mutation containing HsIDH3. (A) α_Mβα_Mγ, (B) α_Mβ, and (C) α_Mγ. These mutant enzymes were measured at a low concentration (1 mg/ml, left panel) and a high concentration (10 mg/ml, right panel). Chromatograms show the molecular weight (MW, black), and the normalized curves of the light scattering at 90° (LS, blue), differential refractive index (DRI, red) and UV (green). All experiments were performed twice, which yielded similar results, and for each case, only the data of one representative experiment is shown. SEC-MALS, size-exclusion chromatography–multiangle light scattering.