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. 2022 Nov 29;66:101646. doi: 10.1016/j.molmet.2022.101646

Figure 2.

Figure 2

Silencing of thyroid hormone receptors results in decreased ChREBPα and ChREBPβ transcription. Ins-1 cells were transduced with lentivirus containing shRNA directed against Thra, Thrb or control shRNA. Following the transduction, Ins-1 cells were cultured for 48 h in RPMI with 10% resin stripped serum with the indicated glucose and T3 concentrations. Thra, Thrb, ChREBPα, and ChREBPβ mRNA levels were determined by qRT-PCR. (A, B) The specificity of each shRNA to silencing its own receptor was tested. Sequence for silencing as well as for qPCR detects both splice isoforms of each respective gene (C–F) The effect of knocking down each thyroid hormone receptors on ChREBPα (C) and ChREBPβ (D), Txnip (E), and Pklr (F) expression was examined. Data are the mean ± SEM of at least three independent experiments. All mRNA levels were normalized to β-actin.∗P < 0.05; ∗∗P < 001; ∗∗∗P < 005; ∗∗∗∗P < 001, compared to control 2 mM glucose within each respective group (0 nM T3 or 10 nM T3). $P < 0.05 compared to control 20 mM within each respective group. Statistical test-two way Anova.