TABLE 1.
First validation set strains for F PCR assay
| F serotype(s)
|
F PCR result | Strain name | O:K:H serotype (or source strain, if clone) | Source or reference | |
|---|---|---|---|---|---|
| Putativea | Confirmatory | ||||
| F7-1, F7-2 | NDb | F7-1, F7-2 | AD110 | O6:K2:H1 | 18 |
| F7-1d | ND | F7-1 | MC400/pDAL201B | (AD110) | 55 |
| F7-2d | ND | F7-2 | HB101/pDAL210B | (AD110) | 55 |
| F7-1, F7-2 | ND | F7-1, F7-2 | CFT073 | O6:K2:H1 | 60 |
| F7-1 | ND | F7-1 | DH5α/pKgg201 | (CFT073) | 60 |
| F7-2 | ND | F7-2 | DH5α/pDIW101 | (CFT073) | 60 |
| F7-2 | ND | F7-2 | C952-79 | O6:K2:H1 | IEKC |
| F7-2 | ND | F7-2 | C953-79 | O6:K2:H1 | IEKC |
| F7-2 | ND | F7-2 | C1898-79 | O6:K2:H1 | IEKC |
| F7-2 | ND | F7-2 | 2H4 | O6:K2:H1 | 41 |
| F8 | F8, F10 | F8, F10 | C1253-77 | O18ac:K−:H− | IEKC |
| F8 | F8 | F8, F10 | C1254-77 | O75:K−:H5 | IEKC |
| F8 | F8, F10 | F8, F10 | C351-82 | O18ac:K−:H− | IEKC |
| F8 | F8, F10 | F8, F10 | C405-82 | O18ac:K−:H5 | IEKC |
| F8 | F8, F10e | F8, F10 | C805-83 | O18ac:K−:H− | IEKC |
| F8 | F− | F10 | C659-81 | O1:K51:H− | IEKC |
| F8 | F8 | F8, F10 | C321-82 | O18ac:K−:H− | IEKC |
| F8 | F8 | F8, F10 | C825-83 | O75:K5:H− | IEKC |
| F8 | F8, F12 | F8, F12 | V30b | O16:K1:H− | 41 |
| F9 | F9 | F9, F10 | 3669 | O2:K5:H4 | 12 |
| F9d | ND | F9 | HB101/pDAL200A | (3669) | 12 |
| F9 | F9 | F9, F10 | C1018-79 | O2:K5:H4 | IEKC |
| F9 | F9 | F9, F10 | C481-82 | O1:K1:H− | IEKC |
| F9 | F9, F10e | F8, F9 | C483-82 | O1?:K1:H− | IEKC |
| F9 | F14, F16 | F13, F14, F16 | C878-83 | O4:K12:H5 | IEKC |
| F10 | ND | F10 | C1960-79 | O7:K1:[H1] | IEKC |
| F10 | ND | F10 | C997-79 | O7:K1:H− | IEKC |
| F10 | ND | F10 | C328-82 | O7:K1:H− | IEKC |
| F10 | ND | F10 | C906-83 | O7:K1:H− | IEKC |
| NAc | F− | F10 | G1062a | O4:K7:HR | 38 |
| F11 | F11, F16e | F11, F16 | IA2 | O4:K12:H− | 10 |
| F11 | ND | F11 | HB101/pDC1 | (IA2) | 16 |
| F11 | ND | F11 | C1976-79 | O1:K1:H7 | IEKC |
| F11 | ND | F11 | C974-79 | O1:K1:H7 | IEKC |
| F11 | F10e, F11 | F10, F11 | V31 | O4:K12:H− | 41 |
| F11, F16 | ND | F11, F16 | AFR015 | O4:K+:H− | 45 |
| F11, F16 | F10e, F11, F16 | F10, F11, F16 | BOS040 | O4:K+:H1 | 45 |
| NA | F10, F11, F16 | F10, F11, F16 | JR1 | O4:K7:H1 | 82 |
| F12-1, F12-2 | ND | F12 | C1979-79 | O16K1:H− | IEKC |
| F12-2 | ND | F12 | C493-82 | O6:K13:H1 | IEKC |
| F12-2 | ND | F12 | C438-82 | O75:K5:H5 | IEKC |
| F12-2 | ND | F12 | C469-82 | O75:K95:H5 | IEKC |
| F13 | ND | F13 | J96 | O4:K−:H5 | 44 |
| F13 | ND | F13 | DH5α/pJJ48 | (J96: allele I) | 47 |
| F13 | ND | F13 | HB101/pJFK102 | (J96: allele III) | 51 |
| F13 | F13, F14 | F13, F14 | CP9 | O4:K10,K54/96:H5 | 44 |
| NA | ND | F13 | NM554/pCP9I | (CP9) | This study |
| NA | ND | F14 | NM554/pCP9III | (CP9) | This study |
| F13 | F13, F14 | F13, F14 | BF1023 | O4:K10,K54/96:H5 | 44 |
| F13 | F13, F14 | F13, F14 | BF1056 | O4:K10,K54/96:H5 | 44 |
| F13 | F13, F14 | F13, F14 | BOS038 | O4:K10,K54/96:H5 | 44 |
| F13 | F13, F14 | F13, F14 | BOS110 | O4:K10,K54/96:H5 | 44 |
| F13 | F13, F14 | F13, F14 | 518 | O4:K10,K54/96:H5 | 45 |
| F13 | ND | F13, F14 | 18878 | O4:K10,K54/96:H5 | IEKC |
| F13 | ND | F13 | BF1040 | O4:K3:H5 | 44 |
| F13 | ND | F13 | BF9043 | O4:K3:H5 | 44 |
| F13 | ND | F13 | CA002 | O4:K3:H5 | 45 |
| F13 | ND | F13 | CA062 | O4:K3:H5 | 45 |
| F13 | ND | F9, F13 | CA022 | O4:K3:H5 | 45 |
| F13 | F11,e F13 | F11, F13 | R28 | O4:K3:H5 | 45 |
| F13 | F13, F16 | F13, F16 | 3048 | O4:K−:H− | 31 |
| F13, 16 | ND | F13, 14, 16 | C134-73 | O4:K12:H5 | IEKC |
| F13, F14, F16 | ND | F13, F14, F16 | 20025 | O4:K12:H− | IEKC |
| F14 | ND | F14 | C127-86f | (Unknown) | IEKC |
| F14 | ND | F14 | C 818-83 | O25:H9 | IEKC |
| F14 | ND | F14 | C1023-79 | O83:K24:H31 | IEKC |
| F15 | ND | F10, F15 | C1805-79 | O75:K5:H− | IEKC |
| F15 | F− | F10 | C826-83 | O75:K5:H− | IEKC |
| F15 | F10e, F15 | F10, F15 | C372-82 | O75:K5:H+ | IEKC |
| F15 | F10, F15 | F10, F15 | C394-82 | O75:K5:H− | IEKC |
| F15 | F10e, F15 | F10, F15 | C312-82 | O75:K5:H− | IEKC |
| F16 | ND | F16 | C83-83 | O157:K−:H45 | IEKC |
| F16 | F14, F16 | F14, F16 | PM8 | O4:K12:H− | 41 |
| F16 | F16 | F7-1, F16 | BOS021 | O4:K+:H− | 45 |
| F16 | ND | F16 | BOS046 | O4:K12:H− | 45 |
a
According to records of the IEKC.
b
ND, not done.
c
NA, not applicable.
d
F type according to reference 12.
e
Antigen detected only in second or third round of confirmatory serotyping.
f
Recombinant.