TABLE 2.
Second validation set of strains for F PCR assay
| Straina | Published O:K serotype | Confirmatory O:K:H serotype | Published F type | Confirmatory F serotyping result
|
F PCR result | ||
|---|---|---|---|---|---|---|---|
| Round 1 | Round 2 | Round 3 | |||||
| AD303 | O25:K− | O4:K2:H1 | F11, F16 | F11 | F10, F11 | F10, F11, F16 | F10, F11, F16 |
| AD309 | O77:K− | O77:K16:H4 | F7-1, F11, F12 | F11, F12 | NDd | ND | F11b |
| AD314 | O4:K12 | O4:K12:H1 | F11, F16 | F11 | F10?, F11 | F11, F16 | F11, F16 |
| NS3 | O14:K+ | OR:K?:H6 | F7-1, F13, F14 | F7-2, F13 | ND | ND | F7-2, F13 |
| NS24 | O1:K1 | O1:K1:H7 | F7-1, F11 | F11 | ND | ND | F11 |
| NS25 | O4:K12 | O4:K12:H1 | F11, F16 | F11 | F10, F11, F16? | F10, F11, F16 | F10, F11, F16 |
| NS26 | O83:K24 | O83:K−:H31 | F7-2, F14 | F14 | ND | ND | F14 |
| SP57 | O157:K− | O157:K−:H45 | F16 + ? | F− | F10?, F16 | F16c | F16 |
| SP88 | O157:K− | O157:K−:H45 | F16 + ? | F− | F10?, F16 | F16 | F16 |
a
Sources of strains and serotypes: AD309, AD314, NS3, NS24, NS25, and NS26, reference 18; SP57 and SP88, reference 68.
b
Subsequently found to be PCR positive for the new F48 papA allele (but still PCR negative for F12).
c
A second colony type noted in the serotyping laboratory was serotyped as OR:K−:H45, F10, F16. (This variant was not retested with the F PCR assay.)
d
ND, not done.