TABLE 4.
Adhesin genotypes and phenotypes of three control strains positive for F10 by PCR but not by serology
Strainb | O:K:H serotype | F serotype
|
PCR | Adhesin genotypea
|
MRHA pattern | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Putativec | Confirmedd | papAH | papC | papEF | papG | papG allele | afa/draBC | sfaS | ||||
C659-81 | O1:K51:H− | F8 | F− | F10 | + | + | + | − | − | + | − | Non-P |
G1062a | O4:K7:Hr | NAe | F− | F10 | + | + | + | + | III | − | − | P |
C826-83 | O75:K5:H− | F15 | F− | F10 | + | − | − | − | − | − | − | −f |
As determined by PCR, except for papAH in strain C826-83 (PCR negative, probe positive for papAH). +, wild type; −, mutant.
Strains C659-81 (alias FS09) and C826-83 (alias FS30) were from the IEKC laboratory. Strain G1062a (alias C892-97) was from the J.R.J. laboratory (Table 1).
Based on historical serotyping results for strains from the IEKC.
Including with 10× concentrated F10 antiserum.
NA, not applicable (strain G1062a was first serotyped during the present study).
−, HA negative.