Figure 1.

Primary, well-differentiated HAE form inflammasomes and secrete IL-1β but not in response to SARS-CoV-2 infection

(A) IL-1β in pooled HAE supernatants following treatment with indicated stimuli ± transfection (TFX).

(B) IL-1β and IL-18 in HAE supernatants following stimulation.

(C) IL-1β in HAE supernatants following SARS-CoV-2 infection.

(D) Viral titer from HAE apical wash following SARS-CoV-2 infection.

(E) Viral N RNA expression relative to ACTB.

(F) IL1B and IL18 expression relative to ACTB.

(G) Apical IL-1β secretion from HAE following SARS-CoV-2 infection or stimulation.

(H) Viral titer from HAE apical wash following infection.

(I) LDH in HAE apical wash following SARS-CoV-2 infection. Data points represent individual HAE donors with bars shown as mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, or ^∗∗∗∗p < 0.0001 calculated by ordinary one-way (A and I) or two-way (B and D) ANOVA followed by Dunnett’s (A and I) or Tukey’s (B and D) multiple comparison test.

See also Figure S1.