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. 2022 Dec 9;7:388. doi: 10.1038/s41392-022-01253-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Knocking down CRL4^CUL4A/DDB1 stimulates mitophagy by inducing mitochondrial Parkin translocation in cisplatin-resistant OCCs. a The colocalization of mitochondria and autophagosome was determined by immunofluorescence staining. Briefly, A2780CP cells were observed 24 h after CRL4^CUL4A/DDB1 was knocked down, followed by staining with GFP-LC3 plasmids for 24 h and staining with MitoTracker Red for 30 min. Scale Bar:10 μm. b Graphs show the Pearson’s R values using whole cells by Image J. (n = 4–6 fields). c Immunoblotting analysis of LC3 in the cytosolic (Cyto) and mitochondrial (Mito) fractions of A2780CP (upper panel) and COC1/DDP (lower panel) cells with or without CRL4^CUL4A/DDB1 knockdown. d Western blot analysis of Parkin, PINK1, DDB1, and CUL4A protein levels in A2780CP and COC1/DDP cells with or without CRL4^CUL4A/DDB1 knockdown. e, f Immunoblotting analysis of Parkin and PINK1 in the cytosolic (Cyto) and mitochondrial (Mito) fractions of (left panel) A2780CP and (right panel) COC1/DDP cells with or without CRL4^CUL4A/DDB1 knockdown. g Immunofluorescence analysis of the colocalization of endogenous Parkin and COX4 in A2780CP cells with or without CRL4^CUL4A/DDB1 knockdown. Scale Bar:10 μm. h Immunofluorescence analysis of the colocalization of endogenous COX4 and PINK1 in A2780CP cells with or without CRL4^CUL4A/DDB1 knockdown. Scale Bar:10 μm