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. Author manuscript; available in PMC: 2022 Dec 9.
Published in final edited form as: J Invest Dermatol. 2018 Jul 19;138(12):2531–2539. doi: 10.1016/j.jid.2018.06.186

Figure 1. Jet injection of HSP70iQ435A-encoding DNA can suppress nuclear expression of the HSP in perilesional vitiligo skin.

Figure 1.

Fluorescent protein ZsGreen expression from an expression plasmid was compared between (a) untreated skin, (b) jet injected and (c) microneedle introduction of similar amounts of DNA followed by tissue homogenization after 96 hours and fluorocytometry. (d) Significantly reduced nuclear expression of HSP70i was observed in n = 4 perilesional samples of DNA compared to vehicle-treated skin, comparing epidermal expression before (t = 0) and at the end (t = 3 weeks) of treatment. (e) Example of reduced nuclear HSP70i expression. Scale bar = 25 μm. HSP70iQ435A, inducible HSP70Q435A.