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. 2022 Nov 25;49(4):248–258. doi: 10.5653/cerm.2022.05358

Figure 2.

Figure 2.

(A) Colony polymerase chain reaction for the confirmation of HCG gene cloning in the plasmid expressing green fluorescent protein-N1 (PEGFP-N1) vector. Lane 1: DNA ladder (1 kb). Lane 2: positive clone (670 bp). (B) Enzymatic digestion of the PEGFP-N1-HCG vector (recombinant vector) by BamHI and EcoRI. Lane 1: DNA ladder (500 bp) and Lane 2: the lower band (560 bp) is the HCG gene, and the upper band (4,700 bp) is the PEGFP-N1 vector. (C) The enzyme-linked immunosorbent assay method was applied to determine the concentration of produced HCG in the different groups. HCG, human chorionic gonadotropin; PBMC-pE, transfected PBMC with empty pEGFP-N1 vector; PBMC-pE-HCG, transfected PBMC with pEGFP-N1-HCG vector.